Spectramax gemini fluorescent 96 well
WebEnzyme-Linked Immunosorbent Assay (ELISA) is a technology used to quantitatively notice an antigenic within a sample such as peptides, proteins, antibodies and hormones, dungeon lysates, blood samples, and food items. WebA fluorogenic substrate for detection of organophosphatase activity
Spectramax gemini fluorescent 96 well
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WebInstruments with a 96- or 384-well format will accommodate the most commonly used plates. However, for greater flexibility, some instruments can accommodate 6- to 1536 … WebPlants are rich in secondary metabolites, which are often useful as a relevant source of nutraceuticals. Quercetin (QUE) is a flavonol aglycone able to bind Transthyretin (TTR), a plasma protein that under pathological conditions can lose its native
WebApr 13, 2024 · The substrate was added and fluorescence was measured for 120 min every 2 min (λ excitation = 320 nm; λ emission = 420 nm) using a SpectraMax Gemini EM microplate reader (Molecular Devices). WebAID 1343835 - Enzymatic Assay: Furin activity was measured in triplicate in wells of a 96-well plate in 0.2 ml 50 mM HEPES, pH 7.5, containing 1 mM CaCl2, 0.005% Brij-35 and 20% glycerol. Pyr-RTKR-AMC (25 μM) was used as a substrate. The concentration of furin in the reactions was 50 nM.
WebMicroplates The SpectraMax GEMINI EM can accommodate standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Black-walled, clear-bottom or all black microplates are generally recommended for fluorescence assays because they have lower backgrounds than clear plates. Page 17: The Optical System WebThe 96-well plates were then tested using a spectrophotometer (SpectraMax M3, Molecular Devices) at a wavelength of 570 nm to obtain the optical density. Cell density was …
WebMay 5, 2024 · Briefly, 96-well plates were coated with basement membrane extract (BME) solution provided by the kit for 30-60 minutes at 37°C, and the cells stained with Calcein AM for fluorescent monitoring of the tubes. iPSC-ECs were seeded at 3x10 4 cells per 100 μL EBM2 medium per well in pre-coated 96-well plates. Vehicle (DMSO) and other top ...
WebITI=::Industrial Technology Institute of Sri Lanka , The pioneer Scientific Research & Development organization in Sri Lanka.We are the successor to Ceylon Institute of Scientific and Industrial Research ( CISIR ) and comes under the purview of the Ministry of Science, Technology and Research and is accredited as per ISO 17025:2005 and conforms to ISO … book of the month log inWebThe top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Other features and benefits include: Eliminate changing out filters Measure more accurately Avoid losing high signal god\u0027s word teachesWeb0.5/ well: Luminescence Performance: Wavelength Range: 250-850nm: Detection Limit: 10 amol/well Alkaline Phosphate 200 µl/well: General Specifications: Microplate Formats: 6-, … book of the month giftsWebThe top-reading Gemini XPS Microplate Reader with dual monochromators lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for your new assays. Multiple-point well … book of the month march picksWebThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Protect your initial investment — purchase a system with the flexibility to add novel detection capabilities without the need for service engineers or costly system downtime. god\\u0027s word speaks to usWebexpressing Green Fluorescent Protein (GFP) in a 96-well microplate using the SPECTRAmax GEMINI-XS spectrofluorometer. MATERIALS AND METHODS Human embryonic kidney … book of the month march 2023WebSpectraMax Gemini EM (Manufacturer: Molecular Devices) Key Features Reads a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and … god\u0027s word teaches us how to live